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A major part of my research works has been and continue to be articulated around observations of the endothelial cell monolayer, intercellular crosstalks, the communications with their environment and other cell types such as immune cells. Here you are some fun videos of my research.
Transmigration of blood monocyte under flow through endothelial cell-cell junctions
Combination of light and fluorescence microscopy (monocytes and endothelial cell monolayer in phase contraste, endothelial cell junctions highlighted by VE-cadherin staining in fluorescence (Credit: A. Sidibé)
Here, flowing monocytes are captured on endothelial cell surface, they crawl to endothelial cell-cell junctions where they transmigrate.
Cite this as: Sidibé et al Nature Communications 2018
Recruitment of monocyte subpopulations by the inflamed endothelium
Recruitment of monocyte subpopulations by the inflamed endothelium
Two populations of monocytes are captured by the endothelium, the classical inflammatory monocytes (not stained) crawl to cell junctions and transmigrate whereas the non-classical patrolling and angiogenic monocytes are captured but remain crawling on the surface of the endothelium. These cells are excluded from extravasation due to CX3CL1 expression on the surface the endothelium.
Tracking monocyte capture, crawling and transendothelial migration under flow
Blood monocytes flowing over an inflamed endothelium are captured by endothelial surface molecules (i.e. Selectins, chemokines, adhesion ligands) that induce the mononuclear cell crawling to cell junctions where they arrest due to activation of integrins and transmigrate to the abluminal face of endothelial cells. (Credit: A. Sidibé)
Cite this as: Sidibé et al Nature Communications 2018
Transmigration of monocyte subpopulations by the endothelium
Transmigration of monocyte subpopulations by the endothelium
Special inflammatory conditions are needed for non-classical monocytes to transmigrate. This requires the reduction of CX3CL1 expression on the surface the endothelium. Thus, non-classical monocytes become sensitive to chemokines triggering transmigration. (Credit: A. Sidibé)
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